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A pseudogene is a variant that has been annotated as having lost its protein coding function, due to, for example, a frame shift.

Multiple pea aphid proteins have been annotated as having the enzymatic activity DOPA decarboxylase (EC 4.1.1.28) in AcypiCyc and, among them, the gene ACYPI009626 received the highest annotation score [ 56].

Another six proteins have been annotated as having the enzymatic activity aromatic-L-amino-acid decarboxylase (EC 4.1.1.28) but with a lower score [ 56]: five of them did not show any change in gene expression during development, while one showed a reduction in expression in the comparison LE vs. L1 (Additional file 10: Table S11A).

If the number of Chinese fir genes is assumed to be commensurate with that of Populus trichocarpa (black cottonwood), which has been annotated as having 45,555 genes [ 55], then our annotated Unigenes represent 59.76% of the number of black cottonwood genes.

To test this we examined the overlap between the subset of C. albicans genes whose expression was induced in vivo (Table 2) and the subset of C. albicans genes that are essential for virulence (i.e. those genes that have been annotated as having an impact upon virulence by the Candida Genome Database: www.candidagenome.org) (Fig. 6; Supplementary data).

Six out of eight genes which have introns in their 5' UTR according to our RACE results have also been annotated as having introns in the TAIR database, and similarly five out of seven genes which have introns in 3' UTR have also been so annotated in the TAIR database.

Similar(54)

Those probes not meeting the criterion for annotation were annotated as having unknown function.

Among the 657 sequences with domain annotation (Table S1), 497 (75.6%) are annotated as having a single domain, 138 (21.0%) two domains, and the remaining 22 (3.3%) three or more domains; 111 sequences (16.9%) are annotated as all-inclusive domains (>99% of residues within one or more domains).

As for GO molecular function annotation, CA7, IL6R, and SPP1 are annotated as having gelatinase activity; AQP8, SPIB, and TCN1 are annotated as being capable of binding; GUCA2B is annotated as a regulator of enzyme activity, and CWH43 is not annotated.

e. start and end of translated region), the information is used to translate the splice pattern sequence onto protein sequence; such a derived protein sequence is annotated as having mRNA experimental evidence; it is often the case that such annotated mRNA entries are associated with protein sequence entries in UniProt [ 28] database.

We also found isologs of phaA (bktB, atoB) encoding additional acetoacetyl-CoA thiolases but these were annotated as having a different substrate specificity than PhaA (Table S9).

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