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Our study provided direct evidence that cloned cells can be transplanted back into animals without being destroyed by the body's immune system.
The idea, eventually, is to generate enough healthy stem cells from a patient's skin that can then be transplanted back into the patient's own body to repair defective organs – such as heart, eyes or muscles – damaged by the mitochondrial mutations inherited from the mother.
The US firm Advanced Cell Technology has used stem cells to regenerate a patient's dying retinal cells, which could be transplanted back into their eyes.
Organoids derived from patients with hereditary diseases can be "cured" with genome editing to correct the disease-causing mutation(s), and functional organoids can then be transplanted back into patients to improve tissue functionality.
If all this were achieved, these cells, in theory, could be transplanted back into the same patient.
Without a validated procedure, regulatory agencies would not allow testicular cells/tissues to be transplanted back into the patient's body.
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But to complete their maturation into sperm, which have half the number of chromosomes of nongerm cells, these PGC-like cells were transplanted back into mouse testes.
The hybrid tooth bone construct was prepared by combining tooth bud cell-seeded scaffolds with autologous iliac crest bone marrow derived stem cell-seeded scaffolds, which were transplanted back into surgically created mandibular defects in the same minipig.
After two days, the cubes were transplanted back into the patients, with the 13 eligible women getting from 40 to 80 pieces each.
The corrected mouse iPSCs were then differentiated into HSCs that were transplanted back into the lethally irradiated mouse model to cure the disease [ 106].
More recently, irradiated haemopoietic stem cells were observed to produce a bystander response in vivo when these were transplanted back into animals (Watson et al, 2000).
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Justyna Jupowicz-Kozak
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