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We recently reported that nucleated erythroblasts matured in vitro can be efficiently infected by P. falciparum [10].
Consequently, receptor masked cancer cells cannot be efficiently infected by progeny CRADs when these are eventually released upon cell lysis.
This cell line can be efficiently infected by the Vero cell-adapted IBV [17] and is much more resistant to the vaccinia virus-induced morphological changes.
We have previously demonstrated that in vitro matured, nucleated erythroblast cells at the orthochromatic stage can be efficiently infected by the human malaria parasite Plasmodium falciparum.
Surprisingly, one of our lung adenocarcinoma cell lines (ADK153) was found to be efficiently infected by MV while displaying a lower expression level of CD46 receptor.
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These data indicate that MEFs are efficiently infected by Mo- and HE-ECTV.
We observed that only 16HBE cells (human bronchial epithelial cells) were efficiently infected by SARS-CoVpp, with levels of luciferase activity similar to those measured in the positive control cell line VeroE6 (Figure 1A).
Nevertheless, ECs can be efficiently infected through co-culturing with Akata cells (an EBV-producing B lymphoblastoid cell line), which is thought to be mediated by a mechanism termed cell-to-cell infection.
Importantly, a significant portion of cells expressing the dn Eps15 mutant were efficiently infected as indicated by yellow fluorescence (Figure S3, right column).
In this assay, the macrophages were efficiently infected (controls = 50% of infected macrophages, with 4.3 parasites/infected cells).
Although the 9L/LacZ rat glioma cell line is infected with relatively low efficiency and thus, lacZ is only partially silenced, this cell line is an appropriate model to study oncolytic HSV-mediated silencing, as silencing is expected to be particularly useful in tumours which are less efficiently infected by the virus and thus, not completely killed by oncolytic HSV treatment.
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