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In order to estimate effective migration rates and divergence times between neighbouring breeding stocks in a stepping-stone fashion (Stock A vs. Stock B, Stock B vs. Stock C, Stock C vs. Stock X) we used a maximum-likelihood framework based on coalescence theory, implemented in the programs MDIV and MIGRATE 2.0.3 [69], [69].

First, alternative connections between haplotypes were broken following a series of rules based on coalescence theory [ 90].

We investigated the demographic profiles of chicken populations based on coalescence theory and analyzed pairwise mismatch distribution to confirm the population expansion [ 39], using Arlequin.

Estimators of migration rates based on coalescence theory can detect asymmetries (directionality) in migration rates and differences in population sizes, a considerable advantage when testing migration rates in the context of evolutionary theory [ 91].

In this study we used a multilocus approach and the Isolation-Migration model based on coalescence theory to estimate the time of divergence of the Spanish and Eastern imperial eagle sister species.

New likelihood and Bayesian methods based on coalescence theory have recently been developed for estimating species divergence times, potentially overcoming some of the above handicaps [e.g. [ 1, 11, 12]].

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We simulated the spatially explicit neutral model in infinite area [27] with simulation procedures based on coalescence as described in [29].

Finally, Bayesian maximum-likelihood method based on coalescence implemented in CoNe [ 40] was employed.

The analyses are based on coalescence, but to increase clarity estimates of m 1 and m 2 are reported in forward-time.

This conclusion, however, is based on coalescence estimates with considerable variation and a small number of comparisons, and should, therefore, be interpreted with caution.

Evidently caution is needed when considering the IM migration estimates and other parameters given that they are based on coalescence simulation of a single locus.

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