Exact(2)
Total RNA from the bacteria was isolated using the TRI reagent method (Sigma, St . Louis MO) and treated with the Turbo DNA-free DNase kit (Ambion, Austin, TX).
Genomic DNA from inactivated preparations of C. burnetii isolates and from non- Coxiella bacteria was isolated using the High Pure PCR Template Preparation Kit™ (Roche Diagnostics, Mannheim, Germany) according to the manufacturer's instructions.
Similar(6)
Six bacteria were isolated using the LB, WA, and DI1 media.
Bacteria were isolated using trypticase soy agar (TSA) and selective agar thiosulphate citrate bile salts–sucrose agar (TCBS) for isolating Vibrio sp. The colony-forming unit (CFU) from fifth time serial dilution (10−5) was selected for colony counting.
Bacteria were isolated using different mediums at proper temperatures after preparation.
Bacteria were isolated using a modified serial dilution method of Maltseva and Oriel [ 25].
It also enabled us to obtain a better understanding of the mechanisms underlying interactions between AMF and bacteria and to follow-up on previous work in which bacteria were isolated using osmotic pressure (Cruz et al., 2008).
Salmonella bacteria were isolated using a routine method [ 36]: briefly faecal samples were enriched aerobically in peptone buffer at 37°C for 24 hours followed by incubation in Rappaport-Vassiliadis broth at 42°C in aerobic conditions again for 24 hours, before inoculation onto Rappaport-Vassiliadis agar and incubation for 24 hours at 37°C.
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