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Typically, variants of a particular locus are provided as different target sequences, and the presence of the variant in the data set being interrogated is revealed by a successful assembly outcome.
Further increasing the sequence reads beyond 300 had very little effect on assembly outcome.
The assembly outcome from dataset 1 attains the longest average read length and N50, while that from dataset 3 yields the most number of transcripts and total base pairs (Table 2).
(Far right) A typical assembly outcome for polymer length 1200 (twice L eq ∗ ) is compared to an EM image of CCMV proteins assembled around an RNA which is twice the CCMV genome length (image extracted from panel C of Figure 5 in Cadena-Nava et al., 2012 ).
DOI: http://dx.doi.org/10.7554/eLife.00632.011 The assembly outcome depends on polymer length, with successful capsid formation occurring when there is overcharging, meaning that the negative charge on the polymer exceeds the net positive charge on an assembled capsid (300 e for this model).
The Assembly outcome was disappointing for the Lib Dems last time too, with their presence reduced from three seats to two.
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With SOLiD assembly as baseline, the assembly outcomes for adding 454 data stabilized at 25x coverage depth and at 200x for Illumina.
With Illumina assembly as baseline, the assembly outcomes for adding 454 data stabilized at 25x coverage depth and at 110x for SOLiD.
Our work showed that amount of sequence data influenced the assembly outcomes in certain range, and increased coverage depth beyond this range seemed to make very little impact.
In a second set of assemblies (Additional file 1) we investigated the effect on Velvet-Oases assembly outcomes of reducing the size of the input data with a digital normalization procedure.
Some isoforms reconstructed by Trinity with the same "chrysalis component" and "butterfly sub-component" had only small variations, such as SNPs, small insertions or deletions; such variations introduced redundancies for the assembly outcomes.
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Justyna Jupowicz-Kozak
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