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All MTT assay were repeated twice.
MTT assay were done in triplicate.
Three replicates of each assay were performed.
Lifespan assay were conducted as described previously [15].
SSF and amylase assay were carried out as described earlier.
At least three replicates for each assay were performed.
Rapid plasma reagin and Treponema pallidum hemagglutination assay were negative.
Reaction conditions of the LAMP assay were tested and optimized.
For validation, matrix-matched calibration and recovery assay were applied.
The reaction temperature and time for this assay were optimized.
Three replicates of each assay were performed and germination data were statistically analyzed using SPSS software.
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