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Plasma was collected in both heparin and citrate buffers and used according to the assay requirements.
Critical assay requirements, including rapid assay, active toxin detection, sensitive and accurate detection, still remain challenging.
Results were promising, but assay requirements prevent widespread application in larger trials.
Samples were further aliquoted based on specific assay requirements and stored at −80°C until use.
Depending on the assay requirements, the level of accuracy can be selected by increasing or decreasing the number of repeats.
Second, QFI-PCR offers absolute quantification that can be used independent of other methods to calculate a minimum copy number input to satisfy downstream assay requirements.
Similar(49)
Positive control varied according to assay requirement.
In all the different antioxidant assays, same dilutions of sample and standards were used; while standard alter per assay requirement.
In all the different antioxidant assays, same dilutions of sample and standards were used; while standard altered as per assay requirement.
TSP employs a biphasic PCR mechanism that simplifies the design of single-marker SNP genotyping assays, reduces requirements for individual assay optimisation, and increases assay specificity and genotyping accuracy.
Analytical performance depends on establishing assay design requirements, identifying key reagent components, and optimizing test procedures and manufacturing processes.
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