Assay condition was the same as in Fig. 1B.
The residual activity was measured under standard assay condition.
At different intervals, samples were withdrawn and the residual activity was measured under standard assay condition.
At intervals predetermined, samples of the enzyme were withdrawn, and their residual activities were measured under the standard assay condition.
Enzyme activity was determined in presence of various concentrations of ethanol (5 30 %) under standard assay condition.
The optimized assay condition was validated according to ICH guidelines to confirm specificity, linearity, accuracy and precision.
The sample concentration providing 50% inhibition (IC50) under the assay condition was calculated from the graph of inhibition percentage against sample concentration.
Enzyme activity was determined in presence of xylose concentration (20 200 mM) under standard assay condition Table 4 Kinetic analysis of β-xylosidase.
In (a), the optimum temperature (open circle) was determined by incubating the enzyme under the standard assay condition at each temperature.
One unit of α-L-arabinofuranosidase activity is defined as amount of enzyme required to release 1 μmol of p-nitrophenol per minute under assay condition.
One unit of filter paper activity is defined as amount of enzyme releasing 1 μmol of glucose per minute under assay condition.
