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The short-lived sonication disperses aggregated materials to LUV.
This allows the investigation of highly aggregated materials or large membrane fragments.
Here, the colluvial soils, though stony, comprise loosely aggregated materials derived from earlier landslide failures, and as such contained no impediments to root development.
In previous studies, the formation of the SCC was observed after exposure of macrophages to fairly high concentrations of potentially aggregated materials, such as acetylated LDL, microcrystalline cholesterol, latex, gold, or polystyrene beads.
Most of the early studies exposed cells to high concentrations of potentially aggregated materials, such as acetylated LDL, microcrystalline cholesterol, hydrophobic latex beads or polystyrene particles, although the degree of their aggregation was not measured in any of these studies.
Cells or other insoluble materials (debris, crystals and aggregated materials) were cleared from the samples by centrifugation at low speed (2500 rpm, 1 h at 4°C), then 5 ml urine sample aliquots were transferred in polypropylene tubes, lyophilized, and stored at -80°C until their usage.
Similar(54)
Aggregated material on filters was detected using anti-polyQ and anti-Sup35 specific antisera.
PrPSc is extracted from affected tissue as highly aggregated material that is not amenable to high-resolution structural techniques.
For in vitro aggregation assays, purified fusion proteins were subjected to ultracentrifugation (100,000 g, 20 min) to remove aggregated material.
To study the formation of fibrillar structures in vitro, purified MBP-fusion proteins were subjected to ultracentrifugation (100,000 g, 20 min, 4°C) in order to remove preformed aggregated material.
The labeled protein was then loaded onto a 2-mL DE52 DEAE cellulose (GE Healthcare, Piscataway, NJ) gravity column to remove both reactive and hydrolyzed forms of free dye as well as aggregated material.
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