Test fish were diverted from the fish return and held for 96 h for observation.
Test fish of groups S - XL were mildly immobilized with Flaxedil (gallamine triethiodide; Sigma-Aldrich, Vienna, Austria) diluted in a Ringer solution.
Test fish were 1-2 years old and reproductively active.
Test fish were naïve with respect to the cave phenotype.
Test fish were then placed in a holding cradle in a Faraday cage.
Test fish were measured for total length (TL) and WM after the tests were completed to not stress the fish.
Test fish came from large, randomly out-bred stocks that were maintained in aerated and filtered 150 200-l aquaria at 27–29°C.
Test fish were transported to the experimental set-up in their isolation tank, thus reducing stress by leaving the fish in its familiar habitat.
I test fish for doneness with a skewer or thin-bladed knife.
The test it uses takes only a minute, Micro Analytical Systems said, allowing it to test fish before it reaches the consumer.
The state conservation department regional director, Ray E. Cowan, declined to appear before Mr. Guldi's committee or to test fish samples collected by Mr. McAllister.
