Sentence examples for DNA amplifier from inspiring English sources

NinjaPCR is the name of a DNA amplifier that is supposed to cut down the costs associated with polymerase chain reaction (PCR), the process of cloning DNA in vitro, by up to 90%.

Targets are captured through joint hybridization of multiple probes, and the complex is detected by signal amplification through a branched DNA amplifier and chemiluminescence signal production.

In a typical biological laboratory routine, many tests are run in parallel by a single equipment; for instance, a thermal cycler or DNA amplifier can perform several different PCR reactions simultaneously.

PCR was carried out on a Tercik DNA amplifier (DNA Technology, Russia) by using DreamTaq PCR Master Mix (Fermentas, EU), under the following conditions: 1 cycle at 94°C for 10 min, 25 cycles at 94°C for 45 sec, 54°C for 45 sec, 68°C for 2 min, 1 cycle at 68°C for 7 min, and a final soak step at 4°C.

PCR for 16S 23S rRNA gene cluster was carried out on a Tercik DNA amplifier (DNA Technology, Russia) by using DreamTaq PCR Master Mix (Fermentas, EU), under the following conditions: 1 cycle at 94°C for 10 min, 25 cycles at 94°C for 45 sec, 54°C for 45 sec, 68°C for 2 min, 1 cycle at 68°C for 7 min, and a final soak step at 4°C.

In situ hybridization protocols, nonspecific hairpin binding studies, in silico and in vitro analysis of DNA HCR amplifiers, characterization of background and signal in situ, probe sequences, and HCR amplifier sequences.

To capitalize on this development, we engineered new high-gain DNA HCR amplifiers for use in permissive hybridization conditions.

Indeed, in stringent hybridization conditions, DNA HCR amplifiers engineered to operate in permissive conditions produced no detectible signal in situ.

The multiplexed in vitro validation study of Figure 3 demonstrates four of these DNA HCR amplifiers operating simultaneously and orthogonally in permissive hybridization conditions.

The performance demonstrated here within intact zebrafish embryos suggests the suitability of next-generation DNA HCR amplifiers for use in diverse imaging settings as well as for diverse applications in vitro.

To decrease reagent cost and improve reagent durability, we set the hard constraint that RNA probes and amplifiers be replaced by new DNA probes and amplifiers engineered to be suitable for use in situ.