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However, in the case of the Y271A mutant, although gapped DNA discrimination was similar to that of the wild-type enzyme, a high degree of gapped DNA enhancement was also observed when the substrates were either of the C10 S BPDE-adducts (dA and dG).
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Cleavage of the probe by RNase H in the presence of its target DNA caused enhancement of donor fluorescence, but this was not observed with nonspecific target DNA.
However, the presence of ss-circular/linear DNA showed enhancement in the ATPase activity of PfRuvB1.
Once again, these results are consistent with a topoisomerase II adduction mechanism for DNA cleavage enhancement by etoposide quinone.
The reason for this increasing 785 cm−1 band intensity could be a local DNA concentration enhancement due to the nucleus fragmentation and the corresponding increased DNA condensation.
Several control reactions were conducted to ensure that the DNA cleavage enhancement observed with etoposide quinone was mediated by topoisomerase IIβ.
Based on these data, we speculate that in men we see a compensation for higher ROS-induced DNA damage via enhancement of DNA repair.
At low DNA concentrations, the enhancement was 80-fold.
After binding to DNA, the notable enhancement was observed.
The H-bonding/hydrophobic force mediated interactions allow the sensing of all three deformed DNA via an enhancement of fluorescence signal using our simple "Just-Mix and Read" strategy.
Upon DNA binding, fluorescence enhancement is observed in the presence of either or both of the amino groups, likely because of more efficient fluorescence quenching through solvent interactions of free amino groups than when buried within the intercalation site.
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