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After inoculum culture, 2 ml of inoculum culture were added into 250 ml Erlenmeyer flask with 50 ml of every entry of fermentation medium (Table 1), which was incubated at 30 °C and run 200 rpm.
These findings should encourage growers to adopt partial saturation on their flooded floors in order to reduce damage from disease after inoculum has been accidentally introduced.
Inducers (Ni, TETA) were added 30 hours after inoculum, when cell density had reached approx. 5×106 cells/ml.
Gas pressure readings were taken with a digital pressure gauge immediately after inoculum addition and after 24 h.
When isolated from footpad lesions (2 months after inoculum), around 20% of amastigotes remained associated with LAMPs.
It was not possible to detect IL-1ra in the GI tract of mice sacrificed four weeks after inoculum (Table 1).
Similar(53)
For bait plant measurements (heights, leaf numbers, root/shoot biomass, leaf nutrients), a compost tea may have positive effects on plant performance when grown in substrates of shallower depths (5.5 cm), even one year after inoculums are applied.
After the inoculum, the shake-flasks were incubated at 30 °C and 180 rpm for 100 h.
After that, inoculum sizes 5%% was transferred to submerged or solid cultured substrate.
Within 15 min after the inoculum has been standardized as described above, a 150 µL of the adjusted inoculums was added into the 96-well microplate containing 150 µL ZnO in the dilution series, and triplicated for each concentration.
Both the mutant and wild type spirochetes were readily cultured from ear and spleen tissues taken from mice 12 days after the inoculum (data not shown).
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