Our own dataset included only a single individual sampled from this subclade, a zoo sample from an elephant that had originated in Sierra Leone (SL0001 in Fig. 2).
In Kumasi Zoo, fecal samples were collected with plastic foil under trees occupied by Eidolon helvum bats (estimated colony size 300,000).
Amplification efficiencies [ 72] were calculated using cDNA synthesized from a 9 mm RA and from a 9 mm RA-Zoo RNA sample for the target genes [i.e. transcripts of interest (TOI)] identified as differentially expressed in the 9 mm stage microarray analyses, and from a 9 mm RA and a 13 mm RA RNA sample for the candidate normalizer genes.
Postmortem examinations were performed on all four zoo felids, and samples were collected for histologic, immunohistochemical, and virologic analyses.
The company turned out a digestable paint and one of their representatives went around to the Zoo with the sample.
Still, it can be applied to analyze the genomes of wild born individuals in zoos where blood samples are usually taken during routine veterinary check-ups.
The zoo's collection includes samples from many endangered species, but not all.
As the process to reduce the carbonate content can influence δ15N values freeze dried whole-body, tail-muscle, and zoo- and phytoplankton samples were allocated into two groups.
Moreover, the samples from Zoo 1 and 2 (including trunkwash and conjunctiva swabs) were also analyzed for EEHV presence by qPCR.
We further included seven hair samples from zoos and 24 museum samples (skin, dried soft tissue, or teeth, Supporting Information Table SI), including samples of holotypes of djamdjamensis (sample ID 529), ellenbecki (sample ID 528), and matschiei (sample ID 530), and a paratype of ellenbecki (sample ID 567, Fig. 1).
