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Images were obtained using a digital CCD camera under UV illumination, and the fraction of DNA below the constriction zone was quantified using NIH ImageJ 1.38 software (NIH, Bethesda, MD, USA).
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When the endogenous number of active zones is quantified using an antibody to endogenous Brp using the same image-processing strategy, DA1 contains 3849 ± 80 active zones.
The mass transfer of TCE vapors from the vadose zone to the atmosphere was quantified using a vertical flux chamber.
The size of the exclusion zone in the autocorrelation was quantified using the effective radius (ER) as previously described [43].
The total number of BrdU+ or DCX+ cells in the subgranular zone of the dentate gyrus was quantified using unbiased stereological methods.
Land-use intensity was quantified using questionnaires.
Aging was quantified using Fourier Transform Infrared Spectroscopy (FTIR).
Fluorescence was quantified using ImageJ.
Total RNA was quantified using Qbit.
Protein content was quantified using EZQuant (Invitrogen).
RNA was quantified using ultraviolet spectrophotometry.
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