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Each unaffected village in this zone of selection was assigned a unique number, and 2 were randomly selected by lottery.
However, compounds which differentially inhibit the sensitive and resistant strains generate inhibition zones with different diameters and thus a ring-shaped zone of selection between them where only one of the strains has grown (Figure 1B).
Furthermore, using our classification methodology (Figure 3C), we find that the majority of these compounds exhibit potentiated rather than direct selection on resistance (∼90% of the compounds show a zone of selection only in the presence of small amount of a tetracycline, Assay Type II).
To find control farms with Sonali or Fayoumi chickens, the same (1 10 km) zone of selection was used, but the names of the farm owners were drawn from the local upazila (a lower administrative unit in Bangladesh) veterinary office and used as the sample from which to randomly select 3 farms.
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We identified and isolated 30 colonies showing clear zone of selections either for or against resistance.
Similar to pure compounds spotted on the plates, secreted compounds diffuse from the plugs and some create zones of selection against resistance (Figure 3B, S2).
By competing two fluorescently labeled strains on the agar, we are able to observe not only absolute zones of inhibition, but also "zones of selection" in which compounds modulate the ratio between the strains (Figure 1B).
Image analysis is performed as described above, and soil extract colonies with distinct zones of selection around them (green rings) are picked through the overlay and re-struck to isolation.
We could not ascertain from the present genetic data whether the excess proportions of parental genotypes in the hybrid zone were products of selection against hybrids, or consequences of admixture (i.e. Wahlund effects).
Geraldine Peacock, chief executive of Guide Dogs for the Blind Association, blames this discrepancy on traditional trustee boards comprised of men working in commerce: "Men are more likely to apply for the top charity jobs because of the salary levels and their culture and profile is more likely to match the comfort zones of the selection panels," she said.
"This is like the Twilight Zone of presidential nominee selection," Mr. Rath said.
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