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After 48 h incubation period the plate was observed for the zone of hydrolysis around the growth.
The organisms were streaked on plates with the respective screening media and the zone of hydrolysis was observed after 24 h incubation.
Correlating with previous report, B. subtilis showed highest zone of hydrolysis as 21 mm in 8 days at optimal cellulose basal medium (Acharya et al. 2012).
Several xylanlolytic strains were isolated and SJ3, which exhibited a large clear zone of hydrolysis, was selected and retained for further experimental study.
After 24 h incubation period the mycelial growth was observed on the plate, but the zone of hydrolysis was observed after 48 h which confirms the ability of R. stolonifer to produce extracellular chitinase.
The isolates that showed high cellulolytic and xylanolytic activities (indicated by the high zone of hydrolysis after the cellulolytic and xylanolytic screening procedures using Congo Red) were subjected to ethanol tolerance test.
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The zones of hydrolysis were viewed by flooding the plates with an aqueous solution of Congo Red (1 mg mL−1) for 15 min followed by 1 M NaCl for another 15 min after draining the dye.
Bacteria belonging to species of the genera Enterococcus and Stenotrophomonas showed marked endo-glucanase activity on CMC plates (Figures 3C and 4A) but despite that they were able to grow on lignocellulose substrates did not show any noticeable clearing zones of hydrolysis on sugarcane biomass substrates (Figures 3A, 3B and 4A).
Clear zones of hydrolysis around the pET28a- ctpAp transformant appeared on the selective agar plates.
An ideal candidate would be a secreted enzyme for which there is a simple plate-based assay that could measure zones of hydrolysis around a colony.
A clear zone of casein hydrolysis gave an indication of protease-producing organisms.
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CEO of Professional Science Editing for Scientists @ prosciediting.com