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The measurement of the diameter of the zone of growth inhibition of microorganisms around the antimicrobial disks in the antibiogram is frequently performed manually by specialists using a ruler.
Next day, zone of growth inhibition around each well was measured.
After 24 h area of inhibition zone of growth was measured.
Synoxazolidinone A (86) showed a zone of growth inhibition against bacterial strain Corynebacterium glutamicum (6.25 µg/mL).
Antimicrobial activity of each compound was evaluated by measuring the zone of growth inhibition against the test organisms with zone reader (Hi Antibiotic zone scale).
Compounds (80 83) exhibited a zone of growth inhibition against fish pathogenic bacterium Vibrio anguillarum (7.5, 7, 7 mm) at concentration 20 µg/6.5 mm discs.
The antibacterial activity was measured as the diameter of the clear zone of growth inhibition and the results were presented in Table 6.
Plates were incubated for 48 h in 37 °C to investigate the antibacterial activity and halo formation (zone of growth inhibition).
Inoculated medium showing an opaque halo around the zone of growth was considered positive.
Strains showing a transparent halo around the zone of growth were considered positive.
The minimum inhibitory concentration (MIC) is read from where the zone of growth inhibition intersects the strip.
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