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The inoculated plates were incubated overnight at 37 °C and checked for a zone of clearing around each bacterial isolate.
Antimicrobial activity of a peptide is visualized as a zone of clearing around each well after 18 24 hours of incubation at 37°C.
In their test, B. subtilis produced a zone of clearing equal to or greater than 7 mm.
The zone of clearing for BshA knockout USA300 JE2 cells treated with 100 μg of fosfomycin in our experiment is 40 mm.
Conducting an agar overlay experiment, we revealed an inhibited growth of Lactococcus lactis, resulting in a zone of clearing around BAA-2069 (data not shown).
While the published data use an amount of fosfomycin more than 3-fold greater than ours, we point out that our results reach a maximal zone of clearing between 25 and 50 μg, and the zone of clearing at 50 μg is the same as that for 100 or 350 μg.
Similar(43)
A minor change in frameshifting efficiencies can be detected and clearly shown phenotypically in terms of zones of clearing on an agar plate.
Microorganisms capable of degrading a polymeric material are determined by looking for zones of clearing around microbial colonies on agar media that are opaque due to the presence of powdered polymer (Pettigrew and Johanson 1996; Ten et al. 2004).
Zones of clearing around colonies after 18 h at 37°C indicated production of β-hemolysin [24].
Zones of clearing around colonies indicated production of cytolysin.
Arthrobacter strain Rue61a grows on skim milk agar plates and produces zones of clearing around the colonies, indicating extracellular proteolytic activity.
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