Exact(2)
1EBOE.HELIX0 (Table 2) also has a high hydrophobic moment, but is actually an isoleucine zipper derived from GCN4.
We made the constitutive GFP-Lpd dimer by inserting a leucine zipper derived from Saccharomyces cerevisiae Gcn4p; (Tomishige et al., 2002) in between the GFP and Lpd protein sequences [EGFP-GGGY KQLEDKVEELASKNYHLENEVARLKKLVEFGGG-Lpd].
Similar(58)
Correcting for the minor effect of the spacer sequence added between syntaxin and SNAP-25, we obtained LD zippering energy of −10 (±2) k B T for the neuronal SNARE complex, consistent with our previous measurement of −8 (±2) k B T. Similarly, we derived the zippering energy of LDs and their associated energy barriers for the other three SNARE complexes (Table 1).
This behavior is not unique: we have already shown that a peptide derived from the b-ZIP (basic-zipper) motif of the c-jun protein manifested the same helix stabilization upon binding to DNA [65].
Membrane fusion between vesicles and target membranes involves the zippering of a four-helix bundle generated by constituent helices derived from target– and vesicle soluble N-ethylmaleimide sensitive factor attachmeN-ethylmaleimide sensitiveEs).
Based on these considerations, we chose to use a genetic approach based on engineered heterodimerizing coiled-coil protein structures derived from vitellogenin gene binding protein (VBP), a leucine zipper DNA binding protein [4].
Maternal embryonic leucine-zipper kinase, a protein overexpressed in many breast cancers and cell lines derived from such tumours, has been shown to bind and downregulate BCL-GL by phosphorylating it.
Nuclear factor (erythroid derived 2) like 2 known as NFEL2 or Nrf2 is a cap'n'collar (CNC) basic-region leucine zipper (bZIP) transcription factor which plays a major role in countering oxidative stress [ 81– 81].
The zipper?
His zipper was undone.
(d) Examine your zipper.
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