Six newly designed diluents, three cryoprotectants (dimethyl sulfoxide, DMA, glycerol), and five diluent:semen ratios (1 1, 3 1, 6 1, 9 1, and 12 1) were tested.
The five diluent gases are nitrogen, carbon dioxide, chlorodifluoromethane (HCFC-22), 1,1,1,2-tetrafluoroethane (HFC-134a) and 1,1,1,2,3,3,3-heptafluoropropane (HFC-227ea).
Four diluent gases are added on the air-side, either CO2, N2, Ar or a (CO2 + Ar) mixture having the same molar heat capacity as N2.
The difference in measured ignition times for mixtures containing argon and nitrogen as the diluent gas can be attributed to the differing heat capacities of the two diluent species and the level of induction period heat release prior to ignition.
For dilute mixtures of isooctane, calculations have predicted that mixtures with argon will ignite faster than those with nitrogen, based on the relative heat capacities of the two diluent gases.
The 7 µm-thick sections were incubated with anti-bSOLD1 antibody, anti-collagen antibody of type-I, or anti-collagen antibody of type-III (CosmoBio, Tokyo Japan), at a dilution of 1∶100 (anti-bSOLD1) or 1∶20 (anti-collagen) in Ab Diluent (Ventana) for 4 h.
All samples were run in duplicate with five diluent control wells, five negative control wells and two positive control wells.
PFBR refueling is revised to accommodate twelve diluents.
Based on these assessments, two diluents were chosen and used in all successive cryopreservation experiments.
Four diluents (CO2, N2, Ar, and CO2 + Ar) were used in preparation for discriminating between the effects, namely, dilution, thermal action, and chemistry, induced by diluent addition.
