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The MS was operated in Ultrascan mode with a 3 m/ z window.
The m/ z window was 0.005 Da and the intensity threshold was 0.05.
Intensity values for pixels in the MS image are calculated by integrating a small m/ z window within the corresponding mass spectrum.
MS images of a selected analyte peak are generated after the measurement by extracting the signal intensity within a certain m/ z window.
For different lipid classes, the m/ z window was set to be 0.01 or 0.005 while the intensity threshold was set to be 0.005 to 0.05.
The accumulation charge capacity of the downstream LIT is devoted only to the charge transmitted in the selected m/ z window.
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The inherent disadvantages of using denaturing conditions include the following: (a) it is less "native" due to the loss of protein tertiary structure, (b) ion signals are spread out over more species, which reduces S/N, and (c) all ion signals appear in narrower m/ z windows, making undesirable overlap more likely.
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Spectral response of Zhong (Z -window has been used to provide the filtering action.
Z-windows are used as variable windows by varying the side-lobe dip position.
However, the optimal size of m/ z variation window is data dependent in the m/ z variation window approach.
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