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Then write down your list and reverse the process.
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When you return home, just go through the above list and reverse everything.
For each annotation, forward primer sequence is listed first and reverse primer sequence is listed second.
The list of forward and reverse primers used for validation is provided in Additional file 14: Table S8.
†Concentrations listed are for forward and reverse primers separately.
The cDNAs for each nov locus were amplified by PCR using the forward and reverse primers listed Table S1.
ATPase subunit 6 (and and CYb templates were PCR amplified using the forward and reverse primers listed in Table 1 and plasmids described previously [10].
Specific primer sequences (forward and reverse) are listed in Table 1.
The restriction sites and the oligonucleotides used as forward and reverse primers are listed in Additional file 2: Table S2.
Forward and reverse primers are listed in Table S1.
The forward and reverse primers are listed in Table 1.
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