Exact(5)
We constructed an architecture comprising three fluorophores: Pacific Blue (PB), an intercalating cyanine dye (oxazole yellow; YO, 1) and Cyanine 3 (Cy3, Figure 1a).
In this context a 1.5-fold increase in Cy3 emission was observed relative to the equivalent assembly comprising six equivalents of YO (1) (1@DNA80, 6.0 equiv; Figure 2c).
Increasing the number of equivalents of YO (1) from one to three (1@DNA21, 3.0 equiv; Figure 2a, right) resulted in a decrease in PB emission and an increase in Cy3 emission to levels approaching that of the PA-containing assembly 2@DNA21.
In the case of the DNA55 series, a two-fold enhancement in Cy3 emission was observed for the DNA assembly comprising four PA binding sites (2@DNA55) relative to assemblies comprising an equivalent amount of YO (1) (1@DNA55, 4.0 equiv).
This is indicative of the extra energy injected into 1@DNA21 (3.0 equiv) is being trapped within the non-sequence selective YO (1) dyes as a consequence of a heterogeneous ensemble of energy transfer events involving both hetero-FRET (i.e. ET from PB to YO and from YO to Cy3) and homo-FRET (i.e. ET between YO dyes) processes.
Similar(55)
We observed several different ways of representing age such as '24 yrs', '25 yo' and '23 ± 2 years old'.
Two weeks later, behavior was assessed in the elevated plus maze (EPMZ) in rats after i.p. saline or YO (1.0 mg/kg).
Barterhouse Bourbon Whisky, 20 YO (45.1% ABV).
Jennifer Lopez & Gente de Zona – "Ni Tú Ni Yo" – 110 BPM.
The Whoop & Holler 28 YO (42% ABV), is a Tennessee whiskey produced by the George Dickel distillery.
The Rhetoric 22 YO (45.2% ABV) has a deep, dark amber color with traces of orange throughout.
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