It has previously been shown that an orientation yielding an upwards brain current (as used here) generates the greatest MEP suppression.
Through the use of an optimized fluorophore, the bright CFP variant mTurquoise (see Additional file 3), positioned in an orientation yielding a higher basal FRET efficiency level in the intact heterotrimer, in combination with YFP-Gγ2 as an acceptor, we were able to measure Gq activation kinetics in single cells with high temporal resolution.
For example, to image tissues or thicker regions of epoxy-embedded cells, semi-thin sections must generally be produced by ultramicrotomy and then imaged from many different sample orientations, yielding a set of digital electron micrographs.
Given a smooth inertial 3D trajectory to be followed by some vehicle point, the developed algorithm uses the vehicle dynamic model, and computes the 3D corresponding body-fixed linear and angular velocities as well as vehicle orientations, yielding a feasible 6 DOF trajectory.
In the other, the 16 sheets have been placed in such a way that each subsequent sheet was at a 45° angle with respect to the preceding sheet, thereby yielding a quasi-isotropic orientation.
The simulated uniaxial tensile overall stress strain responses of the BMG composites containing different volume fractions, shapes, orientations and yielding stresses of toughening particles are compared with the compressive ones.
In this chapter, we focus on the most important factors that need to be taken into account to optimize nanoparticle biofunctionalization and provide an overview of recent approaches yielding the efficient and orientation-controlled immobilization of complex molecules, including peptides and proteins.
14 Provided a suitable thermodynamic interaction force is present, this is then followed by a slow re-orientation eventually yielding an intercalating state.
DOI: http://dx.doi.org/10.7554/eLife.09248.017 EmrE is one of a small number of dual-topology TM proteins in which a single polypeptide chain can insert into the membrane in two opposite orientations, thus yielding inverted symmetric TMH topologies (Duran and Meiler, 2013).
In other words, NMDA and GABA receptors must be functional at some level, since systemic administration of vinpocetine resulted in the restoration of pCREB to cortical cells indiscriminately, yielding the recovery of cortical orientation selectivity.
