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Figure 3b shows that fermentation at 37 °C yielded the highest flocculating activity reaching 80%% with 2.9 g/L of cell biomass.
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The highest flocculating activity was achieved at 24 h of fermentation with a neutral pH 7.0.
This finding was similar with that by B. mojavensis showing the highest flocculating activity within the same period (Elkady et al. 2011).
In the present study, the use of 5%% (v/v) of B. marisflavi NA8 in POME medium resulted in the highest flocculating activity.
The highest flocculating activity was peaked at the early stationary phase, i.e. 24 h, indicating that biosynthesis was responsible for the production of bioflocculant.
The highest flocculating efficiencies for C. reinhardtii and C. minutissima were 99.04 and 93.83 %, respectively.
Flocculating activities of more than 60% were observed in the pH range assessed with the highest flocculating activity of 79% obtained at pH 11.
Over 85%% flocculating activity was achieved at all the tested temperatures, and the highest flocculating activity of 92.68 % was achieved at 35 °C.
As shown in Fig. 3, the flocculating activities of broth with four kinds of lignocellulosic biomasses were much higher than those of the control without added biomasses, and the highest flocculating activity was achieved when corn stover was used as carbon source.
Therefore, weak alkaline culture condition can improve the degradation efficiency of lignocellulosic biomass, and the highest flocculating activity of 90.88 % was achieved at initial pH 8.2 (Na2concentrationtion was 0.4 g/L).
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