Automated annotation with the SAMS platform yielded annotations for 1,200 contigs (28%), whereas 1,969 contigs (46%) did not produce any BLAST hit below tool cut-off.
Annotation against GO database yielded significant annotation for 31,959 out of 72,220 assembled sequences, representing the best possible hits.
Annotation of 20,387 assembled sequences against GO database yielded significant annotation for 15,810 unique transcripts that were classified into biological processes, molecular functions and cellular component using plant specific GO slims.
However, the last years have shown a dramatic growth in datasets that result from high-throughput experiments and computational work and yield annotation sources that provide manifold information about, for instance, protein interactions, signaling circuits, metabolic pathways, cellular localization, tissue expression, disease associations and protein domain architecture.
Many-to-one ETA yielded 30 annotations, of which 87% were correct, whereas one-to-many ETA made 33 annotations with 85% accuracy.
Our sequencing, assembly, and annotation efforts yielded an annotated transcriptome library for T. bernacchii containing 30,107 unigenes of which 13,003 possess strong homology to known proteins.
This yielded significant annotations representing the best possible hits which were classified into three major categories namely, biological process, cellular component functions and molecular functions.
DAVID analysis on these groups yielded similar annotations consisting of significant biological process terms including terms "pattern specification process", and "gastrulation" (Table S2, S3, S4, S5, S6, S7).
Merging the two annotation strategies yielded 26,726 validated annotations.
Considering only the character annotation component (1467 h) of the FC dataset yields an annotation rate of 1.84 characters per hour.
