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Sequencing of putative SFP-containing amplicons from this region and other positions in the genome yielded a validation rate more than 95%.
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Of the 20 FPs tested, all were confirmed, yielding a validation rate of 100%.
This criterion is likely to yield a high validation rate.
Only six panned out — a validation rate of eleven per cent.
Filtering lowers the number of junctions found while raising the validation rate, so that at a validation rate of 94.5%, SpliceMap detected 121,718 junctions.
The validation of a subset of SNPs by PCR amplification and Sanger sequencing revealed a validation rate over 85%.
Random selection and validation of 24 indels occurring in 6 or more samples revealed a validation rate of 100%.
We have not seen similar large scale qPCR validations for other C. elegans microarray experiments, so we cannot comment on whether this is a common phenomenon; however two other microarray analyses performed in our lab utilizing heat-shock expression of a transcription factor yielded similar validation rates (<50%; J. Siegel and D. Eisenmann; L. Gorrepati, and D. Eisenmann, unpublished results).
Using these two PCs as features, LDA leave-one-out cross validation yielded a classification accuracy rate of 71.9% (specificity: 78.9%; sensitivity: 61.5%) and F-score of 0.769.
Using it as feature, LDA leave-one-out cross validation yielded a classification accuracy rate of 81.3% (specificity: 89.5%; sensitivity: 69.2%) and F-score of 0.850.
Using left CgH volume as feature, LDA leave-one-out cross validation yielded a classification accuracy rate of 65.6% (specificity: 78.9%; sensitivity: 46.2%) and F-score of 0.732.
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