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The introduction of a ring of arginine residues near the constriction in the transmembrane β barrel of the staphylococcal α-hemolysin heptamer yielded a pore that could be almost completely blocked by phosphate anions at pH 7.5.
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The masking by particles yielded a pore geometry which varied from complex shaped channels to small individual pores.
This analysis, in the BET approximation, yields a pore diameter of approximately 3.6 nm (low mesoporosity).
The same sample, filtered through a 0.5 μm pore-size membrane, yielded a MW that was at least one order of magnitude lower.
Uncoupling both Cts from the pore domain of the tandem, 3G 3G, yielded a channel that lacked a robust temperature response (I40/I14=4.67±0.21) and that matched the response of the unlinked K2P2.1-3G K2P2.1-3G
The approach of identifying hinge regions for domain fitting to the suilysin pore map has yielded a pseudo-atomic model that goes beyond the earlier rigid body fitting to pneumolysin (Tilley et al., 2005).
The theoretical analysis yielded an average pore radius for each material of about 5 nm, with a particularly narrow pore-size distribution.
In the former case, Fukunaka's group found that Ni deposition initially yielded a hollow tube in each pore, and resulting in a structure transition from the tube to the wire at the growth front [18].
That yielded a surprise.
This model also confirms the helix-tilt model and yields a pore diameter of 2.8 nm.
That passion has yielded a hefty fortune.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com