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Optimization of extraction conditions with minimum pancreatic lipase activity and maximum yield was determined using response surface methodology with three-level-three-factor Box Behnken design (BBD).
The PL quantum yield was determined using Rhodamine 6G as fluorescence standard.
For about one hour after the initiation of the reaction with the sulfatase, the fluorescence intensity increased in a time-dependent manner (Ф F = 0.146; the quantum yield was determined using tryptophan (Ф F = 0.12 in water) as a standard) (Brouwer 2011).
The fluorescence quantum yield was determined using purified solutions of cmFP512 as reference samples [43].
Quality and yield was determined using Lab-on-a-chip (BioAnalyzer, Agilent).
The RNA yield was determined using the NanoDrop 2000 spectro-photometer.
Similar(47)
After purification with the RNeasy mini kit (Qiagen), label efficiency and yield were determined using a Nanodrop spectrophotometer (Nanodrop technologies).
Quantum yields were determined using a standard integrating sphere (diameter 6 cm) [45].
PL quantum yields were determined using a conventional method by comparison of the integrated fluorescence intensity with that of standard dye solutions.
Approximate DNA yields were determined using a spectrophotometer (model U-2001, Hitachi).
Relative fluorescence quantum yields were determined using rhodamine 101 (Fluka, www.sigmaaldrich.com) as a standard.
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