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One plaque failed to yield sequence, and the remaining eleven sequences of the heptamer peptide ligands for F1-40 are shown, with their frequency of occurrence, in Table 3.
DNA cross contamination can yield sequence heterozygosity but contamination should have affected each gene/region across the entire mtgenome.
The thresholds and parameters were selected to yield sequence datasets of 5 10 million bps, which we found to be optimal for motif discovery.
However, such pipelines are rapidly evolving through the incorporation of new, high-throughput sequencing platforms, that obviate conventional clone libraries, or Sanger sequencing chemistries [ 2, 3], but yield sequence reads of limited length.
Further bolstering AHE loci's utility for phylogeographic studies, we found that AHE loci yield sequence divergence estimates strongly correlated with those from TM data, and inferred divergence histories reasonably consistent with previous population-level studies of intron sequences.
It should be noted that in the time since this data has been generated, both Illumina and Life Technologies have introduced sequencing kits that produce even longer reads than what was used here – both platforms yield sequence reads that are twice as long as what was used in this study.
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Note! PCR-based DNA sequencing strategies may not yield sequencing reads across two Mu ends in inverse relative orientation, possibly due to intramolecular hybridization problems.
The functions σ(T i, Q k ) and τ(T i, T j ; Q k, Q l ) yield sequence-based similarities between elements of target and query in the unpaired and paired part of the consensus structure, respectively.
Samples from locations 13, 15, and 23 did not yield sequences from the nuclear loci.
By merging paired-end reads, the overlapping region between them can also be deployed for correcting sequencing errors and potentially yield sequences of higher quality.
Ambiguous pyrosequencing homopolymer stretches in the assemblies were verified by PCR/Sanger sequencing, which invariably yielded sequence that preserved the open reading frame.
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