Among the five tested conventional methods, bead beater/phenol chloroform based DNA extraction method with STES buffer (BP1 method) gave a yield of DNA with high purity.
Among the five conventional methods, bead beater/phenol chloroform based DNA extraction method with STES buffer (BP1 method) gave a yield of DNA with a high purity and resulted in a higher DGGE band diversity.
Estimated tumor fraction in plasma correlated with the normalized yield of DNA extraction from plasma.
The yield of DNA double-strand breaks was only increased by a factor of 1.55, suggesting that other lesions also contribute to cell killing.
A much higher yield of DNA single-strand breaks/krad was observed in a polA1 strain than was reported before for wild-type E. coli.
From the present work, the initial yield of DNA single-strand breaks was found to be 32.4/genome krad-1 (9.0 eV/break) (A67).
Considering the low yield of DNA in oral liquid and injection samples, nested PCR was used to improve the efficacy of amplification (Figure 1a lane 4,5 and Supplementary Figure S3).
(2) The polA1 mutation (DNA polymerase I) increased the sensitivity of E. coli to killing by gamma irradiation in air by a factor of 2.9, and increased the yield of DNA double-strand breaks by a factor of 2.5.
Thus, if a hydrogen donating species, cysteamine, is added or rigorous anoxia is obtained chemically, then heated cells show the same yield of DNA single-strand breaks as do normal cells under extreme hypoxia.
The yield of DNA single-strand breaks was the same under these two conditions, but was about 2-fold higher than for cells grown in Brain Heart Infusion plus glucose.
When added before X-irradiation, quinacrine enhanced the yield of DNA single-strand breaks for cells irradiated in the absence of air (presumably by selectively inhibiting their repair), but not in the presence of air (A44).
