Sentence examples for yield kit from inspiring English sources

32P-labeled AIE RNA probes were prepared by using T7-MEGAshortscript high yield kit according to the manufacture's instruction (Ambion, Austen, TX).

Isolation of RNA, cDNA synthesis and amplification were carried out as previously described [15] with the following modifications: following the second round of amplification and Sephadex G50 minicolumn purification, cDNA was added to an in vitro transcription kit (Megascript T7 high yield kit, Ambion) as per the manufacturers instructions.

Biotin labeled cRNA was synthesized using the Enzo BioArray High Yield kit (Farmingdale, NY).

This lack of difference led us to select the Epicentre Ampliscribe™ T7 High Yield Kit for the extra in vitro transcription step to decrease expense.

cDNA (1 μg) was used as a template for in vitro transcription and biotin labeling reaction using a BioArray High Yield kit (Enzo Life Sciences, Farmingdale, NY).

Fifteen microgram of labelled cRNA was prepared, essentially as per the standard Affymetrix protocol, from 10 μg of total RNA, using the Superscript II system (Invitrogen) and BioArray High Yield Kit (Enzo Life Sciences Ltd., Exeter, UK), and then hybridized to U133A GeneChips.

A total tRNA mixture from E. coli MRE600 and the in vitro protein synthesis system (RTS 100 E. coli High-yield Kit) were obtained from Roche Diagnostics (Indianapolis, IN, USA).

One hundred microlitres of finger prick blood is mixed with 900 µl L6 guanidine buffer (Severn Biotech, UK) and stored at −80°C until automatic nucleic acid extraction by MagNAPure (Roche) using commercial high-yield kits.

Double-stranded cDNA was purified in Phase-Lock Gel Tubes (Eppendorf AG, Hamburg, Germany) and in vitro transcribed by Ambion's MEGAscript ™ T7 High Yield Transcription kit (Ambion, Inc. Austin, TX, USA) followed by cleanup with RNeasy® RNA isolation kit columns (Qiagen).

The MEGAscript T7 High Yield Transcription kit was purchased from Ambion (USA).

The PCR products were used as DNA template for in vitro transcription using TranscriptAid T7 High Yield Transcription Kit (Fermentas, Thermo Scientific).