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Due to extremely low production of GP-1 and long fermentation time of strain ZX01, this is necessary to improve GP-1 yinld in batch culture.
The input parameters of ANN model were subsequently optimized using the GA formalism for obtaining maximum EPS yield in batch fermentation.
Strain EB243 could produce 20 g/L butanol with a yield of 34% (w/w, 83% of theoretical yield) in batch fermentation without any antibiotics and inducers, thus showed great potential for industrial application.
The initial ethanol concentration (33.6 g/L) increased to only around 40 g/L, resulting in a very low ethanol yield in batch SSF, less than 40% of the theoretical, respectively (see Table 2), while great amount of glucose accumulated.
The protein yield in batch and dialysis reactions using BYL was much higher than that of a commercial Promega WGE preparation, achieving a maximum yield of 80 μg/mL of eYFP and 100 μg/mL of luciferase, compared to only 45 μg/mL of eYFP and 35 μg/mL of luciferase in WGEs.
It is, however, important to keep in mind that the inhibition of the yeast is depending on the sum of inhibiting substances, but the results in the present study suggest that inhibition of the yeast can be ruled out as the sole explanation of the low ethanol yield in batch SSF with the whole pretreated slurry (SSF 1).
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Taking this transition into account, the model can better predict refolding yields in batch and pulse refolding strategies.
Thus, despite partial xylose utilization, SSF of spruce with TMB3400 did not necessarily improve the ethanol yields in batch experiments at 10% WIS.
This yield is considerably higher than the yield found in batch phase (0.17 mol PHB mol−1 carbon substrate, Table 1) and the yield, 0.13 mol PHB mol−1 carbon substrate, obtained by Quillaguamán et al. [ 9] in fed-batch cultures using a defined medium containing glucose as carbon source.
The conversion yield during fed-batch phase of the first fermentation was 0.23 mol PHB mol−1 carbon substrate (Table 1 and Figure 1(a)) which is similar to the yield obtained in batch phase due to the formation of biomass.
> -wrap-foot> μmaximumimum specific growth rate, Y x/s biomass yield determined in batch culture at given temperature (T), sinhib substrate concentration resulting in a decrease of the specific growth rate and/or biomass yield with the particular substrate, H heterotroph, P (facultatively) photoautotroph, n.a.a
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