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Another limitation is that although the methodology itself a consultation meeting was culturally appropriate for collecting the perspectives of these stakeholders, it nonetheless did not yield a transcript that could have been coded qualitatively and that could have supported the written documentation of the consultation.
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For example, primer pairs spanning the exon 6/7 and exon 9/10 splice junctions yielded a transcript abundance of about 25% of control in lrrke03680 homozygous flies, whereas primer pairs spanning the exon 7/8 junction yielded an abundance of 90% of control, and primers spanning the exon 4/5 junction gave a 36% increase in transcript abundance (Fig. 1A).
These were assembled and partially annotated to yield a reference transcript set comprising 71,545 sequences.
The result of synchrony would yield a higher mean transcript amount at the promoter level.
Pol Iα was able to transcribe through the nucleosome to yield a full-length transcript of 178 nt.
Pol Iα was able to transcribe the poly-nucleosomal template (poly-N) processively to yield a full-length transcript of 2496 nt.
Most genes can yield a variety of transcripts through a process called splicing.
cDNA was derived from 200 ng of RNA and was amplified with primers 5'-GTACGCTGTGAAGGCATCAA-3' and 5'-GTTGGTGTTCATCCGCTTG-3' to yield a 90 bp transcript-specific fragment of a highly expressed ribosomal protein gene, [GenBank accession # NM_012423.2] RPL13a.
Specifically, from a total of 137 sequenced clones, we identified 19 unique trypsin-like serine protease sequences, and significantly, the male accessory gland yielded a single transcript that was not found in the other male tissues nor in any tissue from females (sequence #5, Table 2).
Of the much larger genome of P. chrysogenum, cultivation under similar conditions yielded a detectable transcript for only 67%% of the genes.
RT-PCR using a forward primer in exon 1 of Iso1 and a reverse primer in the last exon of Iso1 did not generate a fusion transcript, but a forward primer in GFP and the same reverse primer did yield a GFP-82 fusion transcript.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com