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An shRNA-resistant version of c-Yes cDNA (called R-Yes) was generated by site-directed mutagenesis (QuickChange II XL-Stratagen) of full-length human c-Yes cDNA and inserted into pcDNA.1DV5HisTopo (Invitrogen).
A total score was generated counting all "yes" responses for each study.
A current depressive symptoms binary (Yes/No) variable was generated using the cut-off score of ≥10 [ 30].
Ten times as much could be generated by complete legalization (yes, that would be a BILLION dollars).
To judge whether and if yes to what extent explosive gas vapor)/air mixtures will occur or may be generated during malfunction it is necessary to know the safety characteristic data at the respective conditions.
Per blood product, a qualitative variable (transfusion yes or no) and a quantitative variable (amount of product in units) were generated.
Much knowledge has been generated over the years to arrive at the conclusion that yes, photomutagenicity does exist with the use of chemicals, but how to best test for it will require a sophisticated case-by-case approach.
Finally, yes/no questions were generated to help check readers' understanding of the characters' mental states in the story.
Binary variables were generated for working days per week (i.e. ≤5 days or >5 days), shift work and job satisfaction (i.e. yes or no).
And yes, current US practice is generating ill-will, and that too is a factor to consider in reshaping our policy and the law which governs it.
They're generating jobs".
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com