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Reads were aligned using GSNAP [ 48] with the supplied known splice junction from RefGen_v2 working gene set, with the parameters: −-nthreads 12, −-batch 5, −-max-mismatches 0.05, −-npaths 5, −-quiet-if-excessive, −-novelsplicing yes, −-split-output.
The Tories may now split (yes, they might; contingency planning was underway before polling day) losing its moderates to who-knows-what.The Tories might in time get lucky, and get their own Tony Blair and their own New Tory Party.
Outside my window, now half out of the melting snow, stood a pyramid of well-cured rounds sawed, hauled and stacked two summers before from a dead red fir that was felled mostly to improve the view and, yes, eventually to split, haul and feed to our stove.
To split, yes.
Yes, yes, yes, yes, yes.
Yes, yes, yes, yes.
Yes, yes, oh, yes!
I7: Yes, yes, yes.
Yes, yes.
If Scotland votes yes, the UK will split, and we will go our separate ways for ever".
"The hidden danger in the government's proposal is that it will split the yes vote and sink marriage equality in the same way as the republic referendum.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com