Sentence examples for yes containing from inspiring English sources
Protoplasts were harvested by centrifugation, inoculated into YES containing 1 M sorbitol (regeneration medium) (Kobori et al., 1989), and allowed to regenerate at 30°C for 4 6 hours.
Approximately 15 000 transformants growing on selection plates at 27°C were replica-plated onto YES containing phloxin-B and the plates were incubated at 36 or 34°C.
Emodin's estrogenic properties have been previously demonstrated in a recombinant yeast screening assay (YES) containing ERα; emodin was only two orders of magnitude weaker in estrogen response than the E2 standard [ 12].
Cells were cultured over night in YES containing glucose at 0.5% weight per volume until they reached mid-log phase, concentrated by centrifugation, and visualized using phase microscopy using a Zeiss Axioscop microscope with a Plan NeoFluor 40× objective.
It's often heartbreaking, deals with tough issues like suicide, and yes, contains a few lines of vicious language spewing out of the mouths of pre-teens.
Unless otherwise specified, yeast extract + 225 mg/l of supplements (YES) contains 3% glucose, and Edinburgh minimal medium (EMM) contains 2% glucose [ 61].
Serial dilutions of the wild-type and respective mutants were plated on YES, and YES plates containing FOA. DAPI staining were used to determine the percentage of lagging chromosomes as described previously (Gregan et al. 2007).
Cells were grown to mid-log phase, serially diluted five-fold and spotted onto YES plates or YES plates were irradiated with the indicated doses of UV irradiation using a Stratalinker (Stratagene) or YES plates containing 10 or 12 μg/ml thiabendazole (TBZ), 5 mM hydroxyurea (HU), 7 μM camptothecin (CPT), 0.005% methyl methanesulfonate (MMS) and incubated for 2-3 dats at 32°C.
Transformants were selected on YES plates containing 0.1 mg/mL nourseothricin.
Transformants were selected on YES plates containing 0.5 μg/mL aureobasidin A (Takara).
To select for recombinant double-mutant haploids, cells were arrayed on YES plates containing G418 and nourseothricin and allowed to grow for a further 2 days.
