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Yep, with stakes high, he knows how to shine, he knows how to step up.
Yep, with hemorrhagic red Misfits letters and a front-page infographic warning you not to fuck fat girls in the bathroom.
Yep, with apologies to fan boys all over the world and quite possibly Middle Earth, the buzzworthy Las Vegas alt-rock group didn't name themselves over an undying love of Game of Thrones, Dungeons & Dragons, or anything medieval or fantasy filled.
Cells were released in YEP with 2% galactose for at least 60 min before FLIP.
This was achieved by overnight incubation at 4°C of 45 ml YEP with 300 μl Avidin-Agarose beads (Sigma).
A fraction of the cells (1/200) were used to inoculate fresh YEP with dextrose and subsequent cultures.
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The transformants with pSTRE-LacZ TRP1) pSTRE-LacZ TRP1grown in YEP complemented with 2% glucose and 2% raffinose/2% glycerol, respLG/Zvely.
PLF: Yep, work with any PLFer to get a message to me.
LEAGOLDMAN: Yep! Started with Martha Stewart Martha Stewart a couple of years ago, then Doris Fisher Doris Fisher from The Gap.
Me: "Really?" My Mom: "Yep (again with the yep!).
Yep, lunch with Andy Warhol tanked, and I'd give my eyetooth or left testicle (not both) for another shot.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com