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The morphologically uniform NiO hollow microspheres prepared with yeast template after calcination had an averaged diameter of 1.5 2 μm and the shell thickness of ∼500 nm.
The results showed that the prepared samples have a hierarchical porous structure replicated from the microstructure of yeast template, which combines macropores (1.5 2.5 μm) and thin pore wall (0.2 0.7 μm) composed by mesopore (9.78 nm) and 10 20 nm active material.
This template was not included in our modeling protocol because of its significantly lower sequence similarity, and larger number of gaps (3%) when compared with the yeast template.
Nonetheless, given the abovementioned limitations of using the archaeal template, it is prudent to adopt the ligand-bound eEF1A yeast template, singularly, for modeling other eukaryotic targets.
The remaining four variations were not modeled because they are present within a disordered region at the C-terminus of the yeast template, and hence it is unknown how they may influence the overall structure or binding-interactions.
A total of 40 out of 42 residues involved in domain-domain contacts amongst the three domains are identical (Figure 1) for the two variants and the yeast template (contacts identified using PIC [53]).
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Finally, the predicted structures of Esi0007_0019 and Esi0232_0002 were aligned with the yeast Tom70 template using FATCAT [37], [38].
Marco et al. [64] used a model based on the yeast eEF1A template to assess the ability of a potent inhibitor of protein synthesis (didemnin B) to bind human eEF1A1.
We design a 'nanocrystal-glass' configuration to build a nanoarchitecture by means of yeast cell templates self-assembly followed by the controlled in-situ biomineralization of materials on the cell wall.
The model predictions agree well with lab reactor tests and clearly demonstrate improved transport properties and conversions in multiple layers configuration with additional macropores resulting from the yeast cell templates.
(D ) Gallery of structures of several model organisms, obtained by homology modeling on the yeast structure templates using swissmodel.expasy.org.org
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