Our screen reveals mtr1 + as a new fission yeast regulator of interphase microtubule dynamics.
First, we partitioned the known yeast transcriptional regulators into two disjoint sets.
In yeast, transcriptional regulators typically do not function from downstream positions, but act by binding to upstream activation sequences (UAS) that are positioned within a few hundred base pairs from the transcription start site [ 50, 73].
TimeXNet was able to recover a significant portion of the HOG MAPK pathway, the primary response pathway to osmotic stress in yeast, including regulators, Hog1, Hot1, Pbs2, Ypd1, Msn4, Ste11 and Ste20 (See Additional File 3 for the complete list of predicted genes and interactions).
Although the C. elegans motifs described here are not similar to the binding sites of any of the yeast RPG regulators, it is possible that the overall patterns of protein-protein and protein-DNA interactions are related between the two phyla.
Class I HDAC's (1,2,3 and 8) share homology with the yeast transcriptional regulator RPD3, class II HDAC (HDAC 4 7, 9 11) have similarity to yeast Hda1 gene and class III (SIRTs 1 7) share homology with NAD family of sirtuin proteins [9], [17].
Over-expression of the yeast transcriptional regulator Sterol Uptake 1 (SUT1) gene elevates sterol biosynthesis activity [ 29].
Human class I HDACs includes HDAC1, -2, -3, and -8, which are enzymes similar to the yeast transcriptional regulator Rpd3, generally localized to the nucleus [ 4, 5].
Class I HDACs (HDAC1, -2, -3, and −8) are orthologs of the yeast transcriptional regulator RPD3 and are primarily localized in the nucleus.
