Sentence examples for yeast nitrogen from inspiring English sources
The medium was termed "yeast nitrogen base with casamino acid (YNBC) medium".
Yeast nitrogen broth agar plates were inoculated with bacterial suspensions in mid-logarithmic phase by swabbing the agar surface.
Yeast nitrogen base and PTM1 trace metals solution were purchased from the Shanghai Sangon Bioengineering Co. Ltd ,China.
The histidine, methionine and tryptophan concentrations were reduced to 10% of their original concentration in the yeast nitrogen base.
Yeast nitrogen base was selected as the best nitrogen source when compared to the yeast extract and corn steep liquor.
The minimal medium containing 1% glucose and 0.67% yeast nitrogen base (YNB) without amino acids was used to select the yeast transformants.
Yeast cells were inoculated in YNB glucose (yeast nitrogen base, 7.0; glucose, 10.0 g/l distilled water) and incubated on a shaker (130 rpm, 48 h, 20°C).
Transformants were selected for their ability to grow on MD plates (1.34 % yeast nitrogen base, 4 × 10−5 % biotin, 2%% dextrose) lacking histidine at 30 °C.
For the experiments in bioreactors, unbuffered YNB-xylose (13.4 g L−1 Yeast nitrogen base, 50 g L−1 xylose) was used as media.
Autophagy was induced by transferring the cells to SD-N medium (0.17% yeast nitrogen base, without amino acids and ammonium sulfate, and 2% glucose).
