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yeast medium.
Bacteria were grown in GY (glucose yeast medium) (10 g glucose, 2 g yeast extract and 15 g agar per liter).
C. parapsilosis, and G. candidum were cultured by using Malt Yeast medium and its broth was prepared by dissolving Malt extract (3 g/L), Yeast extract (3 g/L), Peptone (5 g/L), Glucose (10 g/L) in distilled water (up to 1 L) and the pH was adjusted to 6.2.
S. stipitis culture was grown for 48 h at 28°C and maintained at 4°C in YM (yeast medium) agar plates containing 10 glucoselucose, 5 g.L−1 peptone, 3 g.L−1 malt extract, 3 g.L−1 yeast extract and 20 g.L−1 agar.
Yeast cells were grown in batch culture using standard defined, complete synthetic yeast medium with 1 2% glucose or YPD medium.
During the study, the flies were kept on the standard yeast medium at a temperature of 29°C and a photoperiod of 12 h.
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Drosophila culture was performed at 25°C on standard cornmeal-yeast medium.
The flies were raised at 23 °C, 60%% humidity, and 12-h light/dark cycle on standard cornmeal-yeast medium and changed every 3 5 days.
We prepared linezolid-containing (500 μg/ml) fly food (cornmeal-yeast medium) by adding 50 mg of linezolid into 100 ml of liquefied food.
Observation vials contained molasses-cornmeal-yeast medium, with 5 mg of yeast.
All strains were maintained on standard Drosophila cornmeal-yeast medium at 25°C.
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