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Yeast extract and K2HPO4 were found to be the key media components for the growth and fermentation which is revealed from their interaction in both the yeast isolates.
We tested oral yeast isolates of Brazilian HIV-infected individuals receiving antiretroviral therapy for protease secretion and susceptibility to ritonavir and some antifungals.
Two hundred and thirty-four yeast isolates from Greater Mekong Subregion (GMS) countries, i.e., Thailand, The Lao People's Democratic Republic (Lao PDR) and Vietnam were obtained.
Fig. 5 Phylogenetic dendrogram of yeast isolates a P. kudriavzevii OBS1 and b S. cerevisiae OBS2.
Therefore, to test the parameters of stress tolerance of yeast isolates the following experiments were performed.
The antioxidant activity of yeast isolates was assayed using phosphomolybdenum method (Kumaran and Karunakaran 2007).
The sugar utilization ability of yeast isolates was achieved by their sugar assimilation profiling.
Yeast isolates were identified by sequence analysis of 18S rRNA gene.
Further the yeast isolates were evaluated for their pathogenicity and adherence to intestinal epithelial cells.
Table 3 Percent tolerance of yeast isolates at different concentration of human gastric enzymes Sl. no.
Fig. 3 Pathogenicity of yeast isolates and commercial probiotic S. boulardii: a protease activity, b phospholipase activity and c haemolytic activity Fig. 4 Pathogenicity of yeast isolates based on gene amplification: a genomic DNA of the yeast isolates and b amplicons of yeast isolates.
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