Sentence examples for yeast fixation from inspiring English sources
Yeast fixation and fluorescence in situ hybridization (FISH) to detect endogenous TLC1 RNA was performed as described (Pfingsten et al. 2012).
After fixation, yeast were stained with PI, and cells with 1N DNA were sorted by flow cytometry.
Functional expression of T. denitrificans Rubisco in S. cerevisiae, as indicated by ribulose-1,5-bisphosphate-dependent CO2 fixation by yeast cell extracts, was only observed upon co-expression of E. coli GroEL/GroES.
We have shown that a mutator allele of msh2 declines rapidly in frequency in experimental populations of sexually reproducing yeast while nearly hitchhiking to fixation in isogenic asexual populations.
This observation confirmed that expression of a heterologous phosphoribulokinase (PRK) gene is required for in vivo carbon fixation via Rubisco in yeast.
A complement-fixation titer to the yeast or mycelial antigens of >1 32 or a fourfold increase in the titer between acute- and convalescent-phase serum specimens was considered evidence of acute infection.
To yield the observed genomic content biases, the strength of such forces would correspond to effective selection of strength γ ∼ −0.7 for fixation of A/T versus C/G in yeast and γ ∼ −1 in Arabidopsis.
Wild-type and RNA interference (RNAi)+ cells carrying a green fluorescent protein (GFP -tagged Dicer were GFP -taggedately in yeast extract peptone Dicerose, then mixed in equal proportions before fixation.
These dyes are compatible with cross-linking fixatives such as formaldehyde, and fixation typically results in more homogenous labeling of LDs in yeast cell populations (Wolinski and Kohlwein 2008).
Even for selectively neutral mutations, the probability of ultimate fixation is only about one in 25 million for the large global yeast population.
This specimen was submitted to CDC for further testing; there, ID exhibited both H and M bands and complement fixation testing found a titer of 1 8 for histoplasmin and >1 256 for whole yeast.
