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Yeast cell lines were genetically engineered to display Hepatitis C virus (HCV) core antigen linked to gold binding peptide (GBP) as a dual-affinity biobrick chimera.
The YES and YAS assays consist of yeast cell lines in which the human AR and ER have been introduced and coupled with a reporter gene that produces an enzyme.
The yeast cell lines employed for deconvoluting hits included yeast constitutively expressing lacZ gene to eliminate false-positives due to β-galactosidase inhibition and Asc/Caspase-1 expressing yeast to eliminate hits that interfere with a different Caspase or its upstream activator, in addition to original Fas/FADD/Caspase-8-expressing yeast.
The development of cellular models of the disease from yeast, cell lines and primary cultures has led to an improved understanding of HD pathology.
Further tests for potential epoxygenase and acetylenase activities that use linoleic acid as substrate have also been carried out in these transgenic yeast cell lines, by supplementing free linoleic acid in the yeast culture upon the induction by galactose.
However, none of hydroxyl derivatives of common fatty acids, such as ricinoleic acid synthesised from oleic acid, was detected in any of the yeast cell lines expressing CtFAD2 genes, indicating the absence of fatty acid hydroxylase activity in the CtFAD2 enzymes.
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The yeast cell line EGY48-6op-LEU2/2op-lacZ/GPD-Fas-d-S8/10 LETD -TA/CYC1-Caspase10-FLAG/ADH-Fas EGY48-6op-LEU2/2op-lacZ/GPD-Fas-d-S8/10 LETD -TA/CYC1-Caspase10-FLAG/ADH-Fastion EGY48-6op-LEU2/2op-lacZ/GPD-Fas-d-S8/10 LETD -TA/CYC1-Caspase10-FLAG/ADH-Fas
In this application, the yeast cell line, EGY48-6op-LEU2/2op-lacZ/GPD-Fas-d-S8/10 LETD -TA/CYC1-Caspase8-HA/ΔADH1-FADD EGY48-6op-LEU2/2op-lacZ/GPD-Fas-d-S8/10 LETD -TA/CYC1-Caspase8-HA/ΔADH1-FADDrs EGY48-6op-LEU2/2op-lacZ/GPD-Fas-d-S8/10 LETD -TA/CYC1-Caspase8-HA/ΔADH1-FADD
We then applied our method to yeast cell line and breast cancer microarray data and obtained biologically plausible results.
A desaturase-deficient yeast cell line (ole1) was transformed with YEpOLEX plasmids containing the Lca-QPAQ, Lca-SPVE or Lca-GATD ORF, respectively.
GC-MS analyses of fatty-acid methyl esters from a recombinant yeast cell line grown in presence of Cu2+ showed that the encoded protein produced a broad range of monounsaturated products.
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