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The number of samples in each cohort, the year the samples were obtained and the vaccine status for the members of each cohort are summarized in Table 2. Titers of antibodies in serum that neutralized 100 TCID50 of Sabin 2, MEF, SD-98 and isolates SD-99-1, and SD-04 were determined by a micro-neutralization assay [30] and normalized to a pool of 20 positive sera in each test run.
The final model included 4 predictors: season of the year the samples were taken, source of replacement stock, herd size and interaction between herd size and season of the year the samples were taken (Table 2).
The effect of season of the year the samples were taken on ASF seropositivity was modified by herd size.
However, the effect of season of the year the samples were taken on ASF herd seropositivity was modified by herd size.
Our modeling indicated that source of replacement stock and season of the year the samples were taken are important determinants of ASF herd seropositivity in southwest Nigeria.
In the multivariable logistic regression adjusting for other covariates that were significant at P < 0.20 and biologically plausible ones, there was an interaction between herd size and season of the year the samples were taken.
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Comparison of ARM and IRM properties measured in 2012 and 2015 for samples from site 51 (Fig. 7) suggests that the coercivity distribution broadened and softened during the 3 years the samples were stored.
Two children had completed five years of treatment each and one child was on treatment for three years when the samples were collected.
Now, more than three years after the samples were taken at the recommendation of a national expert, the results are in, and some sighs of relief are nearly audible.
The cervical samples were tested for HPV infection years after the samples were obtained and the HPV results did not influence subsequent surveillance or treatment of the women.
The average age of children was 10 years, and approximately half the samples were male.
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