Make sure to follow the following format: XX:XX:XX:XX:XX:XX.
Sex determination is an XX-XY system with a male-determining Y factor located in one L genome in males [ 14].
Sex determination in P. esculentus is a genetic XX-XY system with almost no differentiation between the sex-determining chromosomes [ 15].
Dissection of metamorphs from a crossing experiment confirmed that sex determination is an XX-XY system with the Y confined to the L genome.
Therefore, 12 of the 14 Oryzias species have an XX-XY sex determination system, suggesting that the XX-XY system is dominant in this genus.
The most stable and conserved vertebrate systems are in therian mammals (eutherians and marsupial mammals with a highly conserved XX female: XY male system of male heterogamety), and birds and snakes (with highly conserved ZW female: ZZ male systems of female heterogamety).
P. pacificus also has an XX:XO sex determination system, where males occur at a frequency of 0.1% in standard laboratory cultures [ 42, 43].
All sex-linked markers isolated showed male-heterogametic inheritances, indicating that all six species have an XX-XY sex determination system.
For the completely sex-linked markers, all male progeny had the paternal genotype, while all female progeny had the maternal genotype, indicating that all of these species had an XX-XY sex determination system.
While unsuccessful in linking sex to the female map, we were successfully able to map the trait to linkage group 14 of the male map, suggesting an XX-XY sex determination system.
Our sex-linkage analysis demonstrated that all species had an XX-XY sex determination system, and that the Oryzias marmoratus and O. profundicola sex chromosomes were homologous to O. latipes linkage group (LG) 10, while those of the other four species, O. celebensis, O. matanensis, O. wolasi, and O. woworae, were homologous to O. latipes LG 24.
