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Most strikingly, the designed V146H/L363E dyad exhibited a rate of reactivation of 67±6.0×10−4 min−1 (t1/2 of 1.2 hr) following cyclosarin exposure, an increase in reactivation of ∼33,000-fold relative to wt enzyme (where the wt rate was estimated to be 1×10−5 min−1, the lower limit of rate measurement).
The firing rate declined progressively, such that at 40 weeks PCs fired on average at ∼25% the WT rate.
For HSL1, both in vivo and in the model's simulation, growth rate decreased monotonically with successive deletions, with the null mutant achieving only ~65% of the WT rate.
We had previously identified HSL1 as haploinsufficient, and the growth rate of the tetraploid two-copy mutant (approximately 90% of the WT rate) is comparable with our results for the growth of the heterozygous diploid deletion mutant.
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The assumptions for assessment of the wind farm power production are: p s is constant for every WT in OSWF; WTs rated are same and experience constant wind velocity.
For Wt restrictocin, and mutants with near-Wt rates between 0.1 and ∼1 s−1, k2 cannot be determined without the use of a rapid mixing and quenching techniques.
In particular, for WT the rate at transition-1 is 10 times slower than that at transition-0 state, indicating a 2.3 RT increase at transition-1 barrier compared to transition-0.
In contrast, the TuMV WT deletion rate (0.13 0.15%) was similar to that of the ΔGDD control (0.12 0.14%), indicating that pipo-site deletions are not specific to the viral polymerase.
Fig. 10 FMAC-WT: sum rate comparison of CCE, PP and NBS for multiple rate case (with CSI of Eve) Fig. 11 F-MAC-WT: fairness comparison of CCE, PP and NBS for multiple rate case (with CSI of Eve).
Moreover, the impact of different Csb and different WT power rating are investigated.
However, a higher concentration than what was used for Fe(III) citrate was needed to reach WT growth rates and biofilm current densities (Fig. 3C).
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